Data from: Reference transcriptomics of porcine peripheral immune cells created through bulk and single-cell RNA sequencing

This dataset contains files reconstructing single-cell data presented in 'Reference transcriptomics of porcine peripheral immune cells created through bulk and single-cell RNA sequencing' by Herrera-Uribe & Wiarda et al. 2021. Samples of peripheral blood mononuclear cells (PBMCs) were collected from seven pigs and processed for single-cell RNA sequencing (scRNA-seq) in order to provide a reference annotation of porcine immune cell transcriptomics at enhanced, single-cell resolution. Analysis of single-cell data allowed identification of 36 cell clusters that were further classified into 13 cell types, including monocytes, dendritic cells, B cells, antibody-secreting cells, numerous populations of T cells, NK cells, and erythrocytes. Files may be used to reconstruct the data as presented in the manuscript, allowing for individual query by other users. Scripts for original data analysis are available at https://github.com/USDA-FSEPRU/PorcinePBMCs_bulkRNAseq_scRNAseq. Raw data are available at https://www.ebi.ac.uk/ena/browser/view/PRJEB43826. Funding for this dataset was also provided by NRSP8: National Animal Genome Research Program (https://www.nimss.org/projects/view/mrp/outline/18464).

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GUID 673fd36c-e9a0-4522-b6f1-cb68c54df85b
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dcat_modified 2022-08-10
dcat_publisher_name Agricultural Research Service
harvest_object_id c7770a5f-67ae-4e62-ac62-61e0735587af
harvest_source_id 2c0b1e04-ba48-4488-9de5-0dab41f9913f
harvest_source_title USDA Open Data Catalog