This dataset contains 2-hourly observations of biogenic fluxes of carbon dioxide (CO2), nitrous oxide (N2O) and methane (CH4) measured from a winter wheat crop grown on a mineral soil in the UK treated with different fertilisers. The treatments were: i) inorganic fertiliser; ii) pig slurry and inorganic fertiliser; and iii) pig slurry treated with plasma-induction and inorganic fertiliser. Fluxes of biogenic greenhouse gases (GHGs) were measured over an 83-day period (20/03/2022-13/06/2022) within the winter wheat growing season using automated GHG flux chambers.