The impact of lactose on the gut microbiota of healthy adults was examined, using a short-term, in vitro strategy where fecal samples harvested from 18 donors were cultured anaerobically with and without lactose. Donors represented 3 adult age groups. Data collected include: amplicon sequencing of the V1-V2 regions of the 16S rRNA gene (available in the NCBI Sequence Read Archive associated with BioProject PRJNA883645: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA883645), RT-qPCR of Bifidobacterium 16S rRNA genes, and short-chain fatty acid concentrations. Resources in this dataset:

Resource Title: lactose sample attributes File Name: lactose_sample_metadata.csv

Resource Title: lactose SCFA, pH, and gas production data File Name: lactose_SCFA_data.csv

Resource Title: Total bacterial and Bifidobacterium genus qPCR File Name: lactose_qPCR_all_bacteria_and_bifido.csv