The generic placement of the strawberry leaf blight fungus, formerly known as Phomopsis obscurans has always been subject to uncertainty. These datasets provide the phylogenetic evidence based on four DNA markers (28S rDNA/LSU, ITS, TEF1 and RPB2) that support the establishment of a monotypic new fungal genus Paraphomopsis. Datasets include the single gene sequence alignments for the LSU, ITS, TEF1 and RPB2 markers, and the complete combined phylogenetic dataset and phylogenetic tree files for each single gene and combined analysis. The updated multi-gene datasets and trees for the Diaporthales provide the evidence to distinguish the leaf blight pathogen (Paraphomopsis obscurans) from the taxa associated with leaf blotch (Gnomoniopsis fragariae) and petiole blight and root rot (Paragnomonia fragariae).